Osama M. Darwesh 1,2,*, Islam A. Elsehemy 3, Mohamed H. El-Sayed 4,5 , Abbas A. El-Ghamry 4 , Ahmad S. El-Hawary 4

1Agricultural Microbiology Dept., National Research Centre, Dokki, Cairo, Egypt

2School of Material Sciences and Engineering, Hebei University of Technology, 300130 Tianjin, China

3Department of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo, Egypt

4Botany and Microbiology Dept., Faculty of Science (Boys); Al-Azhar University, Nasr city, Cairo, Egypt

5Department of Biology, Faculty of Science and Arts, Northern Border University (Rafha), Kingdom of Saudi Arabia

*corresponding author e-mail address: darweshosama@yahoo.com | Scopus ID 56128096000

Biointerface Research in Applied Chemistry, Volume 10, Issue 1, 2020, 4811 – 4820, https://doi.org/10.33263/BRIAC101.811820

ABSTRACT

Thermoalkaliphilic actinomycetes enzymes have many important applications in many industrial, biotechnological and environmental aspects. So, the current study aimed to obtain the thermoalkali-enzymes producing actinomycetes. A novel thermoalkaliphilic actinomycete strain was isolated from Egyptian Siwa oasis and identified according to its morphological, physiological and biochemical characters as Thermoflavimicrobium dichotomicum. And then confirmed by phylogenetic analysis and the partial sequence was deposited in GenBank under accession number of KR011193 and name of Thermoflavimicrobium dichotomicum HwSw11. It could produce amylase, cellulase, lipase, pectinase and proteinase enzymes. Also, this strain exhibited anti-bacterial activities against P. aeruginosa and E. coli with inhibition zones of 14 and 20 mm, respectively. Consequently, it has antifungal activity against A. niger, A. flavus and Penicillium notatum with inhibition zones of 17, 14 and 14 mm, respectively. For that, it may be concluded that Thermoflavimicrobium dichotomicum HwSw11 as a novel thermoalkaliphile has a wide range of biological activities against a broad spectrum of pathogenic bacteria and fungi, in addition to produce many enzymes (amylase, cellulase, lipase, pectinase and proteinase). So, this isolate could be applied as manufactory for many industrial, biotechnological and environmental sectors.

Keywords: Antimicrobial activity; Enzymes activity; Thermoalkaliphilic actinomycetes; Thermoflavimicrobium dichotomicum.