Yan Kazakov 1, Aleksey Tarasov 1, Lyudmila Alyoshina 1, Khiena Brainina 1, 2,*

1Ural State University of Economics, 62/45, 8 Marta/Narodnoi Voli St., Yekaterinburg 620144, Russia

2Ural Federal University named after the first President of Russia B. N. Yeltsin, 28, Mira St., Yekaterinburg 620078, Russia

*corresponding author e-mail address: baz@usue.ru | Scopus ID 7004124370

Biointerface Research in Applied Chemistry, Volume 10, Issue 1, 2020, 4893 – 4901, https://doi.org/10.33263/BRIAC101.893901

ABSTRACT

The article discusses the relationship between oxidative stress (OS) and pathological conditions, the possibilities and benefits of estimating OS considering the integral antioxidant activity (AOA) as an OS criterion, and using a simple accessible hybrid potentiometric method (HPM) with a mediator system for AOA monitoring. The results of AOA of blood serum in healthy volunteers and patients with various diseases are presented. Preliminary reference values are found. The lower levels of AOA of blood serum in patients with different diseases in comparison with the control group are observed. The potential mechanisms of changes in the AOA levels and it’s clinical significance are discussed from the position of biointerfaces interplay. With AOA equal or greater than 1.40 mmol-eq l–1 the person is healthy, the range from 0.95 to 1.40 mmol-eq l–1 indicates that the patient is at risk and needs to undergo a further medical examination. When AOA blood serum is below 0.95 mmol-eq l–1, detailed diagnostics and relevant treatment are required. The findings allow suggesting that the approach determine antioxidant/oxidant activity of biological fluids holds considerable promise for monitoring OS; it opens up new opportunities in expanding the use of analytical chemistry in medicine.

Keywords: Oxidative stress; Antioxidant activity; Antioxidants; Hybrid potentiometric method; Cardiovascular diseases; Cancer.